Background/Objective: Low back pain is still a challenge in medicine. Numerous factors can influence this phenomenon; of which congenital anomalies such as Lumbosacral Transitional Vertebra (LSTV) that might cause degenerative changes is one. The purpose of this study was to assess pathological findings in adjacent Vertebra in two groups of patients. Patients and Methods: Considering plain AP Lumbar spine x-ray, 91 cases were allocated in LSTV and 108 non-LSTV groups. In Lumbar MRI, disk degeneration, disk herniation, anterior and posterior osteophytes, facet joint hypertrophy, ligamentum flavum hypertrophy and disk height were assessed in both groups in L1-L2, L2-L3, L3-L4, L4-L5 (TV), L5 (TV)-S1 levels.Results: Generally, by reaching level L4-L5 (TV), frequencies of all variables increase and they drop in the level L5 (TV)-S1 in both groups, regardless of LSTV existence. The frequency of disk degeneration on level L4-L5 (TV) in the group with and without LSTV was 81.3% and 75% (P=0.28). In level L5 (TV)-S1, the frequency of disk herniation between these two groups was 34.1% and 56.5% (P=0.02) and the mean value of disk height was 7.4±2.6mm and 9.5±2.27mm (P < 0.001). Comparison of the mean difference of disk degeneration and herniation between two groups was statistically or clinically significant (0.07 and 0.006). We divided patients in both groups into three different age groups (age≤30y, 30yConclusion: It seems that LSTV increases disk degeneration and hernation in the disk above it and preserves the lower disk from these events and its role is more significant when the patient is young.